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分光光度計的結構與使用方法

发表时间：2019-01-18 17:03

1.分光光度法定義與應用

　　1.1定義:分光光度法是利用物質所特有的吸收光譜來鑒別物質或測定其含量的分析檢測技術.

　　1.2特點:靈敏,精確,快速和簡便,在複雜組分系統中,不需要分離,即能檢測出其中所含的極少量物質.

　　1.3應用:生物<>化學研究中廣泛使用的方法之一,廣泛用于糖,蛋白質,核酸,酶等的快速定量檢測.

2.分光光度計的基本結構和工作原理

　　2.1分光光度計的分類

　　2.2分光光度計工作原理

　　2.3分光光度計的基本結構

　　2.4分光光度法的測量誤差

　　2.5顯色反應及其影響因素

　　2.1分光光度計的分類

分光光度計的分類

　　紅外分光光度計:測定波長範圍爲大于760nm的紅外光區

　　可見光分光光度計:測定波長範圍爲400~760nm的可見光區

　　紫外分光光度計:測定波長範圍爲200～400nm的紫外光區

2.2分光光度計工作原理

　　人眼可見的光只占電磁波譜的很小—部分（400~760nm）

　　它是一種頻率較大的電磁波.電磁波按頻率大小,從頻率zui小的無線電波到頻率zui大的γ-射線排成一列,即組成電磁波的波譜,

上一篇石油産品密度測定的意義

下一篇影響油品低溫流動性的因素有哪些

文章分類：技術知識

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